OUP user menu

Comparative analysis of a biofilm-forming Staphylococcus epidermidis strain and its adhesion-positive, accumulation-negative mutant M7

F. Schumacher-Perdreau , C. Heilmann , G. Peters , F. Götz , G. Pulverer
DOI: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06744.x 71-78 First published online: 1 March 1994

Abstract

We have isolated a stable slime-negative mutant, M7, from the wild-type Staphylococcus epidermidis RP62A by mitomycin mutagenesis. Besides its inability to produce slime in the test tube this mutant differed also in two other properties from its parent strain: it lacked the ability to accumulate on a surface, and it did not produce a 115 kDa and a 18 kDa extracellular protein. In all other tested properties such as initial adherence, growth rate, cell-wall composition, surface characteristics, DNA restriction profile, the presence of a 29 kb antibiotic resistance plasmid, and antimicrobial susceptibility profile, M7 was indistinguishable from its wild-type. The mutant is an important basis for further study of the pathogenesis of polymer-associated S. epidermidis infections.

Key words
  • Staphylococcus epidermidis
  • Plastic infection
  • Adherence
  • Accumulation
  • Slime

Log in through your institution