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Subdivision of Salmonella enteritidis PT 4 by pulsed-field gel electrophoresis: Potential for epidemiological surveillance

N.G. Powell, E.J. Threlfall, H. Chart, B. Rowe
DOI: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06888.x 193-198 First published online: 1 June 1994


Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to pulsed-field profile (PFP) 1 predominated. DNA-DNA hybridization studies demonstrated that a band of approximately 57 kb was associated with the presence of the 38 MDa S. enteritidis‘serovar-specific’ plasmid. It is concluded that PFGE provides a method for discriminating strains of S. enteritidis PT 4 suitable for epidemiological investigations.

Key words
  • Salmonella enteritidis PT 4
  • Pulsed-field gel electrophoresis (PFGE)
  • Subdivision
  • Epidemiology

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