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Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli

Andreas Tauch, Oliver Kirchner, Lutz Wehmeier, Jörn Kalinowski, Alfred Pühler
DOI: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07246.x 343-347 First published online: 1 November 1994


Efficient electroporation of Escherichia coli with plasmid DNA isolated from Corynebacterium glutamicum depends on the use of Mcr-deficient E. coli strains. The transformation frequency increased nearly 800-fold when the Mcr-deficient E. coli DH5αMCR was used instead of E. coli DH5α. We used E. coli strains with different mutations in the methyl-specific restriction systems to show that McrBC-deficiency is sufficient to generate this effect. The results imply that C. glutamicum DNA contains methylcytosine in specific sequences recognized by the E. coli McrBC system.

  • Corynebacterium glutamicum
  • Restriction
  • Modification
  • Methylcytosine
  • Electroporation
  • Mrr system
  • Mcr system

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