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Differential synthesis of glyceraldehyde-3-phosphate dehydrogenase polypeptides in stressed yeast cells

Helian Boucherié , Nelly Bataille , Ian T. Fitch , Michel Perrot , Mick F. Tuite
DOI: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07348.x 127-133 First published online: 1 January 1995

Abstract

Three unlinked genes, TDH1, TDH2 and TDH3, encode the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (triose-phosphate dehydrogenase; TDK) in the yeast Saccharomyces cerevisiae. We demonstrate that the synthesis of the three encoded TDK polypeptides (TDHa, TDHb and TDHc, respectively) is not co-ordinately regulated and that TDHa is only synthesised as cells enter stationary phase, due to glucose starvation, or in heat-shocked cells. Furthermore, the synthesis of TDHb, but not TDHc, is strongly repressed by a heat shock. Hence, the TDHa enzyme may play a cellular role, distinct from glycolysis, that is required by stressed cells.

Keywords
  • Saccharomyces cerevisiae
  • Triose phosphate dehydrogenase (glyceraldehyde 3-phosphate dehydrogenase)
  • Stress response
  • Heat shock

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