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NAD+-glycohydrolase from Streptococcus pyogenes shows cyclic ADP-ribose forming activity

Tadahiro Karasawa, Shin Takasawa, Kiyotaka Yamakawa, Hideto Yonekura, Hiroshi Okamoto, Shinichi Nakamura
DOI: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07720.x 201-204 First published online: 1 August 1995

Abstract

NAD+-glycohydrolase from Streptococcus pyogenes was purified by successive chromatography on CM Sepharose CL-6B, Sephacryl S-200 HR and hydroxyapatite. The purified enzyme possessed synthesis and hydrolysis activities of cyclic ADP-ribose (cADPR), a newly found second messenger for Ca2+ mobilisation, along with cleavage activity of the ribose-nicotinamide bond in NAD+.

Key words
  • Cyclic ADP-ribose
  • Streptococcus pyogenes
  • NAD+-glycohydrolase
  • ADP-ribosyl cyclase
  • Cyclic ADP-ribose hydrolase

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