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Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae

Joakim Norbeck, Anders Blomberg
DOI: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08073.x 1-8 First published online: 1 March 1996


Saccharomyces cerevisiae exponentially growing in basic or 0.7 M NaCl medium were isotopically labelled with 35S-methionine, followed by protein separation and quantification by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) combined with computerised image analysis. The electrophoretic separation resolved about 650 proteins of which 13 displayed significant and at least 2-fold changes in rate of synthesis during saline growth. By sequencing of 2D-PAGE resolved proteins, one of the 8 induced spot, p42.9/5.5, was shown to correspond to the full length (containing the N-terminal extension) product of the GPD1 gene encoding the cytoplasmic glycerol 3-phosphate dehydrogenase. The expression of the TDH3 gene, glyceraldehyde 3-phosphate dehydrogenase, and the ENO2 gene, enolase, decreased during growth in NaCl medium, declines hypothesised to have an impact on the flux to glycerol.

  • Saccharomyces cerevisiae
  • NaCl stress
  • Protein synthesis
  • 2D-PAGE
  • Protein sequencing
  • GPD1
  • Glycolytic enzymes