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Cooperative MetR binding in the Escherichia coli glyA control region

Eva Lorenz , George V. Stauffer
DOI: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08097.x 147-152 First published online: 1 April 1996

Abstract

We determined the relative binding affinity of the MetR protein for wild-type and mutant MetR binding sites 1 and 2 in the Escherichia coli glyA control region. The results show that MetR binding site 1 has a higher affinity for the MetR protein than binding site 2. In addition, the results suggest that binding of MetR to the glyA promoter is cooperative. Mutations that decrease the ability of MetR to bind to either site 1 or site 2 have no significant effect on MetR's ability to bend DNA.

Keywords
  • Cooperative MetR binding
  • DNA bending
  • Kd determination
  • Gene activation
  • Escherichia coli