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Glutathione is an important antioxidant molecule in the yeast Saccharomyces cerevisiae

Duncan W.S. Stephen , Derek J. Jamieson
DOI: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08386.x 207-212 First published online: 1 August 1996

Abstract

The tripeptide γ-l-glutamyl-l-cystinylglycine (glutathione) is one of the major antioxidant molecules of cells and is thought to play a vital role in buffering the cell against reactive oxygen species and toxic electrophiles. We wished to determine the role of glutathione in the protection of the yeast Saccharomyces cerevisiae against oxidative stress. This study shows that glutathione is an important antioxidant molecule in yeast, with γ-glutamylcysteine synthetase (gshI) mutants, deficient in glutathione synthesis, being hypersensitive to H2O2 and Superoxide anions in both exponential- and stationary-phase cultures. Despite this, these mutants are still able to induce adaptive stress responses to oxidants.

Keywords
  • Saccharomyces cerevisiae
  • Oxidative stress
  • Adaptive stress response
  • Glutathione