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MetR-mediated repression of the glyA gene in Escherichia coli

Eva Lorenz, George V. Stauffer
DOI: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08535.x 229-233 First published online: 1 November 1996

Abstract

Inactivation of either of the two MetR binding sites centered at bp —143 and —121 relative to the +1 transcription start site results in reduced glyA-lacZ expression in a wild-type strain below the level seen in a metR mutant. This reduced expression is dependent on the side of the DNA helix MetR binds relative to the RNA polymerase binding site. Thus, a single MetR dimer bound to the DNA may play a physiological role in maintaining appropriate glyA gene expression, functioning as a represser under low MetR conditions.

Keywords
  • MetR-mediated activation
  • MetR-mediated repression
  • DNA bending
  • Escherichia coli