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Construction of GFP vectors for use in Gram-negative bacteria other than Escherichia coli

Ann G. Matthysse, Serina Stretton, Catherine Dandie, Nicholas C. McClure, Amanda E. Goodman
DOI: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08561.x 87-94 First published online: 1 November 1996

Abstract

A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli. These constructs were: pTn3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini-Tn5gfp for making random promoter probe gfp insertions directly into host strains; p519gfp and p519ngfp, broad host range mob+ plasmids containing gfp from a lac and an npt2 promoter, respectively.

Keywords
  • Green fluorescent protein
  • Bacterial promoter probe
  • Transposon
  • Plant pathogenic bacteria