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EDTA induced autolysis in Escherichia coli and isolation of resistant mutants

Mireille Leduc, Rouha Kasra, Howard Singer, Jean van Heijenoort
DOI: http://dx.doi.org/10.1111/j.1574-6968.1984.tb01049.x 137-142 First published online: 1 July 1984

Abstract

Autolysis of Escherichia coli induced by a shock treatment with 10−3M EDTA, pH 6.5 was investigated. Mutants presenting reduced rates of EDTA-induced autolysis were isolated. A remarkable feature of these mutants was their tolerance to penicillin G, cephaloridine and moenomycin. Furthermore, a reduced level of peptidoglycan endopeptidase or N-acetylmuramidase activity was observed. Penicillin-binding protein patterns were unaltered.

Key words
  • E. coli
  • EDTA
  • autolysis
  • mutants

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