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Cloning of the gene coding for staphylococcal exfoliative toxin A and its expression in Escherichia coli

Susumu Sakurai, Hitoshi Suzuki, Isamu Kondo
DOI: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02300.x 63-67 First published online: 1 June 1987

Summary

The gene for the staphylococcal exfoliative toxin of serotype A (ETA) was cloned from the genomic DNA into pUC9 and expressed in Escherichia coli C600. Recombinant plasmid pETA-J3 was recovered from an ETA-producing transformant and the eta gene was shown to be located within a 1.7-kb Alu I fragment. ETA produced by the E. coli transformant, cETA, was indistinguishable from that produced by Staphylococcus aureus, sETA, in its physicochemical and serological properties and exfoliative toxicity. cETA was not retained in the periplasmic space; and more than 97% of the toxin was extracellular.

Key words
  • Exfoliative toxin A
  • Gene cloning
  • Gene expression
  • Staphylococcus aureus
  • (Escherichia coli)