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Investigation of possible homologies between crystal proteins of three mosquitocidal strains of Bacillus thuringiensis

David J. Earp, E. Sally Ward, David J. Ellar
DOI: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02071.x 195-199 First published online: 1 July 1987

Summary

The possible presence of protein δ-endotoxins with homology to a cloned mosquitocidal Bacillus thuringiensis var. israelensisδ-endotoxin was investigated in two other mosquitocidal B. thuringiensis strains. Antiserum raised against the var. israelensis 27-kDa toxic polypeptide cross-reacted strongly with a 27-kDa crystal polypeptide of var. PG14 (serotype 8a:8b, a serotype more usually associated only with lepidopteran toxicity). In addition hybridisation studies using a DNA probe derived from the cloned var. israelensis 27-kDa δ-endotoxin gene located a homologous region on a 72–74-MDa plasmid of PG14. A less toxic strain, var. Kyushuensis, showed neither immunological cross-reaction with the antiserum nor DNA hybridization with the probe.

Key words
  • Bacillus thuringiensis
  • δ-Endotoxin gene
  • (DNA homology
  • Plasmid)

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