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Cloning of a Vero toxin (VT1, Shiga-like toxin I) gene from a VT1-converting phage isolated from Escherichia coli O157:H7

Hisao Kurazono, Chihiro Sasakawa, Masanosuke Yoshikawa, Yoshifumi Takeda
DOI: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02235.x 23-26 First published online: 1 September 1987

Summary

Vero toxin (VT1, Shiga-like toxin I)-converting phages were induced with UV light from Escherichia coli O157:H7 strain 83–1386. A non-toxigenic E. coli C600 strain, lysogenized with a toxin-converting phage (86-02), produced VT1. A phage solution was prepared from the lysogenized E. coli C600 (86-02) strain and the phage DNA was prepared.

EcoRI-fragments of the phage DNA were ligated with EcoRI-digested pBR325ΔTc and it was transformed into E. coli strain MC1061. Transformants with VT1 production commonly contained a 5.1-kb EcoRI-fragment. The restriction map of the EcoRI-fragment was prepared and it was found that a 2.1-kb BamHI-BglII fragment encoded VT1 production.

Key words
  • Vero toxin
  • Shiga-like toxin
  • Escherichia coli bacteriophage
  • (Cloning)

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