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Whole-cell ELISA for typing Neisseria meningitidis with monoclonal antibodies

Hussein Abdillahi, Jan T. Poolman
DOI: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02626.x 367-371 First published online: 1 December 1987

Summary

Large-scale screening of Neisseria meningitidis strains is necessary for epidemiological studies as well as for identifying immunologically important antigens. We have developed a new and simpler type of ELISA for this purpose. Whole bacteria from the strains being studied are coated onto PVC plates; the type and subtype are then determined by the binding of monoclonal antibodies with known specificities, detected with a protein A-conjugate. This technique is rapid and easy but still sensitive and reproducible and is thus highly suitable for screening for antigens in a relatively native state on large numbers of strains.

Key words
  • Neisseria meningitidis
  • Whole-cell ELISA
  • Monoclonal antibodies
  • Serotyping
  • Subtyping

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