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Genome analysis of Pseudomonas aeruginosa by field inversion gel electrophoresis

Dietmar Grothues, Burkhard Tümmler
DOI: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02635.x 419-422 First published online: 1 December 1987

Summary

The genome of Pseudomonas aeruginosa was analysed by digestion with rare-cutting restriction endonucleases and subsequent field inversion gel electrophoresis (FIGE). P. aeruginosa strain PAO and the 17 IATS strains were investigated. Each strain displayed a unique pattern of restriction fragments. Digestion with DraI and SspI yielded, respectively 7–11 and 2–5 fragments of more than 130 kb in size, indicating the non-random occurrence of AT-rich sequences in the P. aeruginosa genome. The genome size of P. aeruginosa PAO was estimated to be (2.2 ± 0.3) × 106 bp. The applications of DNA fingerprinting for gene cloning, construction of a physical chromosome map, and epidemiological studies, are discussed.

Key words
  • Pseudomonas aeruginosa
  • Genome fingerprinting
  • Genome size
  • Field inversion gel electrophoresis
  • (Strain typing)

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