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Identification of a repetitive DNA sequence specific to Mycobacterium paratuberculosis

Desmond M. Collins , Diana M. Gabric , Geoffrey W. De Lisle
DOI: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03440.x 175-178 First published online: 1 July 1989

Summary

A 0.2-kb DNA sequence specific to Mycobacterium paratuberculosis, the causative organism of Johne's disease, was isolated from a partial genomic library. The sequence was part of a larger repetitive DNA element and was present in strains of M. paratuberculosis from cattle, sheep, goat, deer and also a woman with Crohn's disease but not in M. paratuberculosis strain 18. The sequence was not present in strains of 19 other mycobacterial species including 31 reference serotype strains of the M. avium-M. intracellular-M. scrofulaceum (MAIS) complex, some strains of which are closely related to M. paratuberculosis. The sequence may be useful for developing a diagnostic test for Johne's disease.

Key words
  • Repetitive DNA
  • Johne's disease
  • Mycobacterium paratuberculosis
  • Mycobacterium avium

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