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Electroporation of Azospirillum brasilense with plasmid DNA

Ann Vande Broek , August van Gool , Jos Vanderleyden
DOI: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03574.x 177-181 First published online: 1 October 1989

Summary

The feasibility of electric field mediated transformation of the nitrogen fixing bacterium Azospirillum was studied. The broad host range plasmid pRK290 was used throughout this study. Transformants were obtained with all A. brasilense strains tested, although with strain dependent efficiency. No transformants were obtained with an A. lipoferum strain. Transfer of the pRK290 plasmid DNA in the A. brasilense strains was confirmed by DNA extraction of the transformants and gel electrophoresis. The effects of the physiological status of the cells and the electric field strength during electroporation were studied in detail for one particular A. brasilense strain.

Key words
  • Azospirillum
  • Electroporation
  • Plasmid DNA

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