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Simplified procedure for purification of Bordetella bronchiseptica dermonecrotic toxin

Yasuhiko Horiguchi, Toyotsugu Nakai, Katsumi Kume
DOI: http://dx.doi.org/10.1111/j.1574-6968.1990.tb03969.x 39-43 First published online: 1 January 1990


Bordetella bronchiseptica dermonecrotic toxin was purified by a simplified method. The method consisted of SP Toyopearl 650M chromatography and high performance liquid chromatography on a TSK gel G3000SW column. 47.5% of the activity of the crude cell extract was recovered. The purified toxin behaved as a homogeneous protein in sodium dodecyl sulfate polyacrylamide gel electrophoresis, high performance liquid chromatography, and agar gel double diffusion tests.

Key words
  • Bordetella bronchiseptica
  • Dermonecrotic toxin
  • Purification

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