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A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model

Rhona Sloane, Joyce C.S. de Azavedo, John P. Arbuthnott, Patrick J. Hartigan, Barry Kreiswirth, Richard Novick, Timothy J. Foster
DOI: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04449.x 239-244 First published online: 1 March 1991


The gene coding for toxic shock syndrome toxin-1 in S. aureus was inactivated by allelic replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This suggests that TSST-1 is the only determinant produced by this strain that can induce the symptoms of shock in rabbits. A novel method for allelic replacement involving transduction of plasmid integrants is described.

Key words
  • Staphylococcus aureus
  • Toxic shock syndrome toxin
  • Allele replacement mutant
  • Animal model

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