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Partial characterization of an 89-kDa highly immunoreactive protein from Chlamydia psittaci A/22 causing ovine abortion

Roberto Cevenini, Manuela Donati, Emiliana Brocchi, Franco De Simone, Michele La Placa
DOI: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04722.x 111-115 First published online: 1 June 1991

Summary

An 89-kDa immunogen from Chlamydia psittaci A/22 causing ovine abortion was partially characterized. The 89-kDa protein, localized on the outer membrane complex of chlamydiae, was synthesized relatively early in the developmental cycle. The protein contained cysteine but was not extensively cross-linked by disulfide bonds. Treatment with proteases apparently did not cleave the protein. The infectivity of strain A/22 was partially (60%) reduced by treatment of chlamydial elementary bodies with monoclonal antibody BS/89 specifically reacting with the 89-kDa antigen. Species-specific as well as strain-specific antigenic determinants were present on the 89-kDa protein.

Key words
  • Chlamydia psittaci A/22
  • Ovine abortion
  • Protein (89-kDa) characterization

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