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Molecular cloning of the ViaB region of Salmonella typhi

Yasuhiro Hashimoto, Takayuki Ezaki, Na Li, Hiroyuki Yamamoto
DOI: http://dx.doi.org/10.1111/j.1574-6968.1991.tb05124.x 53-56 First published online: 1 December 1991


The ViaB region required for Vi antigen production in Salmonella typhi was cloned. The plasmid pGBM124 containing a 14-kb S. thypi chromosomal DNA fragment conferred the ability to produce Vi antigen on Escherichia coli HB101 and ViaB-deleted S. typhi GIFU10007-3. Tn5 insertion analysis showed that the 14-kb DNA was split into three regions. Region 1 and region 2 are involved in the biosynthesis of Vi polysaccharide. Region 3 is involved in translocation of the Vi polysaccharide to the cell surface. Southern blot hybridization showed that regions 2 and 3 but not region 1, were considerably homologous to the DNA of Vi-positive Citrobacter freundii.

Key words
  • Salmonella typhi
  • Citrobacter freundii
  • Vi antigen, ViaB locus
  • Cloning

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