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Gene probes for the detection of 6-deoxyhexose metabolism in secondary metabolite-producing streptomycetes

Michael Stockmann, Wolfgang Piepersberg
DOI: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05149.x 185-189 First published online: 1 January 1992


DNA probes were designed from the streptomycin production genes strDELM of Streptomyces griseus involved in the biosynthesis of the 6-deoxyhexose (6DOH) dihydrostreptose which could detect the genomic fragments coding for 6DOH formation in other actinomycetes strains. In about 70% of the 43 strains tested at least one signal could be detected with strD-, strE- or strL M-specific probes. Evidence is presented that the hybridizing genes are mostly clustered and probably engaged in the formation of secondary metabolites. Because of the wide-spread use of 6DOH constituents in natural products these probes should allow to detect a vast array of different secondary metabolic gene clusters in actinomycetes.

Key words
  • 6-Deoxyhexose metabolism
  • DNA probe
  • Streptomycete

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