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Clonal differences within M-types of the group A streptococcus revealed by pulsed field gel electrophoresis

Leigh A. Single, Diana R. Martin
DOI: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05188.x 85-89 First published online: 1 February 1992

Summary

Digestion of chromosomal DNA with the rare cutting restriction enzyme Sfi I in association with pulsed field gel electrophoresis was used to observe restriction fragment length polymorphisms (RFLP) among isolates of group A Streptococcus. Streptococci examined included isolates belonging to the same M-type (epidemiologically related and unrelated), and isolates from other M-types. RFLP patterns were quite distinct between all serotypes tested. More importantly, isolates from within a serotype could be differentiated by this technique.

Key words
  • Phylogenetic
  • Restriction fragment length polymorphism
  • Epidemiology

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