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The PurR binding site in the glyA prometer region of Escherichia coli

John G. Steiert , Christopher Kubu , George V. Stauffer
DOI: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05585.x 299-304 First published online: 1 December 1992

Summary

Site-directed mutagenesis was used to change the PurR binding site in the control region of a glyA-lac gene fusion. Mutations that changed the PurR binding sequence away from the consensus sequence reduced PurR binding, which correlated with reduced purine-mediated repression. Mutations that changed the binding sequence toward the consensus sequence had no significant effect on either PurR binding or purine-mediated repression. Hypoxanthine and guanine, co-repressors for PurR-mediated regulation of the pur regulon, increased binding of PurR to glyA operator DNA.

Key words
  • glyA operator
  • PurR repressor
  • glyA-lac Fusion
  • Negative regulation
  • Escherichia coli

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